Journal: bioRxiv
Article Title: NEUROMUSCULAR ELECTRICAL STIMULATION LIMITS MUSCLE WEAKNESS, ATROPHY, MODULATES SATELLITE CELL FUNCTION AND REDUCES INFLAMMATION IN CANCER CACHEXIA
doi: 10.64898/2026.04.24.720589
Figure Lengend Snippet: A) Representative dot plots. B) Proportion of neutrophils ( i.e. , CD64 neg Ly6C pos cells) among the CD45 positive cells. C) Proportion of macrophages ( i.e. , CD64 pos cells) among the CD45 positive cells. D) Proportion of macrophages ( i.e. , CD64 pos cells) expressing high (Ly6C pos ), intermediate (Ly6C int ) or low levels of Ly6C (Ly6C neg ). E) Proportion of macrophages expressing CCR2. Data are expressed in relation to the CD64 positive cells. For each FACS experiment, 2 to 4 muscles were included from each group (n = 4 replicates for each group). F) Immunostaining for laminin (grey), CD64 (red), CD169 (yellow) and Hoescht (cyan) on gastrocnemius muscle section from PBS, C26 and C26 NMES mice. Scale bar: 50 µm. G) Number of macrophages in PBS (n=8), C26 (n=9), and C26 NMES (n=8) mice. H) Proportion of macrophages positive for CD169 in PBS (n=8), C26 (n=9), and C26 NMES (n=8) mice. I) Immunostaining for dystrophin (grey), CD169 (yellow), Ki67 (purple) and Hoescht (cyan) on gastrocnemius muscle section from PBS, C26 and C26 NMES mice. Scale bar: 50 µm. J) Number of resident macrophages expressing CD169 in PBS (n=11), C26 (n=12), and C26 NMES (n=12) mice. K) Number of proliferating resident macrophages expressing CD169 in PBS (n=8), C26 (n=8), and C26 NMES (n=8) mice. L) Study design: bone-marrow derived macrophages (BMDMs) from healthy BALB/c mice were exposed to proteins extracts from PBS, C26 and C26 NMES muscles. Inflammatory status was analyzed by immunofluorescence after 48h post-exposure. M-P) Proportion of macrophages positive for COX2 (M), TNF α (N), CD206 (O) and Il-10 (P) and representative immunostaining. Scale bar: 50 µm. Data were obtained from 4-5 different protein extracts per group. *,**,*** P< 0.05, P< 0.01 and P< 0.001, respectively. Values are reported as mean ± SD.
Article Snippet: Ten-week-old BALB/c male mice were purchased from Janvier-Labs (Le Genest-Saint-Isle, France) and were housed individually in a humidity- and temperature-controlled facility (12:12-h light cycle, 25°C).
Techniques: Expressing, Muscles, Immunostaining, Derivative Assay, Immunofluorescence
